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RESPONSE OF STREAM BIOFILMS TO PULSED VERSUS STEADY-STATE PHOSPHOROUS ADDITIONS

Our current understanding of how algal-dominate biofilms in streams respond to phosphorus (P) enrichment is largely based on the assumption that streams have a constant P supply. However, in reality natural streams experience large swings in P concentrations due to runoff and in-stream biotic and abiotic uptake. The purpose of this study was to compare the effects of a steady-state P release versus successive pulse events on algae-dominated biofilms colonizing artificial streams. One treatment (n=4) was maintained at a constant 12 µg P/L, another was subjected to weekly 8 h pulses at 252 µg P/L (n=4) and a third treatment was maintained below P detection limits (n=4). Both the steady-state and the pulse treatments received an equivalent amount of P by the end of the experiment. Preliminary pulse amplitude modulation fluorometry data indicate that algae treated with the phosphorous pulse had a greater photosynthetic capacity and ability to utilize the phosphorus.

Aaron Gordon-Weaver (Primary Presenter/Author, Co-Presenter/Co-Author), Bloomsburg University of Pennsylvania, amg43366@gmail.com;


Jennifer Tuomisto ( Co-Presenter/Co-Author), Bloomsburg University, jat18435@huskies.bloomu.edu;


Steven Rier ( Co-Presenter/Co-Author), Bloomsburg University, srier@bloomu.edu;