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SIMULIIDAE (DIPTERA) CYTOSPECIES COLLECTED FROM BEDROCK SUBSTRATE STREAMS THROUGHOUT OHIO.

Black fly larvae and pupae were collected from first and second order streams with shale bedrock substrates from northeastern Ohio, limestone bedrock substrate streams from southwestern Ohio, and shale bedrock streams from south central Ohio, all within different ecoregions. The sampling occurred from March through June over several seasons; species were identified using cytological methods. Simulium claricentrum (Diptera: Simuliidae), a new state record, was collected in far northeastern Ohio within Ashtabula and Lake Counties. Shale bedrock streams south or west of these two counties did not contain S. claricentrum. Additionally, multiple year sampling of streams containing S. claricentrum did not reveal S. innoxium, which commonly occurs in association with S. claricentrum in Pennsylvania. S. parnassum, a species not previously known to occur in Ohio was collected in a shale bedrock stream in Adams County of southcentral Ohio. Black fly diversity was highest in the northeast shale bedrock streams and the lowest in the limestone bedrock streams. This work is part of an on-going statewide comprehensive cytological survey of Simuliidae in Ohio.

Mike Mendel (Primary Presenter/Author), Cedarville University, mmendel@cedarville.edu;


Peter Adler ( Co-Presenter/Co-Author), Clemson University, padler@clemson.edu;


Gregg Mendel ( Co-Presenter/Co-Author), Cedarville University, gwmendel@cedarville.edu;


Rhonda Mendel ( Co-Presenter/Co-Author), EnviroScience Inc., rmendel@enviroscienceinc.com;